Pantoea ananatis lmg 2665t synthesizes and utilizes acyl homoserine lactones ahls for signalling. A diagnostic multiplex pcr scheme for identification of. During the period from december 2014 to july 2015, 40 isolates ofpantoea spp. Strains of pantoea ananatis tested for the presence of t6ss gene clusters. Bacterial diseases of onion were a limiting factor for yields in 2012 for growers in allegan and ottawa counties. Pantoea ananatis is a ubiquitous organism found in almost every environment on earth. By contrast, its sister species, pantoea stewartii subsp. Previous in silico analyses described three different t6ss loci present in the pathogenic strain of p.
The ecological diversity of this species may be attributed, in part, to the acquisition of mobile genetic elements. Pantoea agglomerans is a gramnegative bacterium that belongs to the family erwiniaceae it was formerly called enterobacter agglomerans, or erwinia herbicola and is an ubiquitous bacterium commonly isolated from plant surfaces, seeds, fruit, and animal or human feces and can be found throughout a honeybees environment. The complete set of genes regulated by the eanir quorum sensing qs system in this strain is still not fully known. Frontiers pantoea ananatis genetic diversity analysis. Genome sequence of pantoea ananatis sgair0210, isolated. Pantoea ananatis is a gramnegative, motile, facultative anaerobe belonging to the gamma proteobacteria. However, it is not a legal authority for statutory or. Genome sequence of pantoea ananatis sgair0210, isolated from.
Integrative conjugative elements of the icepan family play a. Transmission of pantoea ananatis, causal agent of center rot of onion, by tobacco thrips, frankliniella fusca. Its genome was estimated at 5,102,640 bp with 4,994 coding sequences, encompassing genes related to the metabolism of carbohydrates, to the synthesis of auxins, siderophores, and homoserine lactones, and to the type i, ii, iii, iv, and vi secretion systems. Pdf isolation and identification of pantoea ananatis from onion. Final performance report agricultural marketing service. Analysis of the pantoea ananatis pangenome reveals. Isolation of pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion article pdf available in genetics and molecular research. Initially, it formed part of the erwinia herbicolaenterobacter agglomerans complex and was assigned to the genus pantoea when it was established.
This bacterium can survive and multiply in a variety of ecological niches as a. Pantoea agglomerans, a bacterium associated with plants, is not an obligate infectious agent in humans. Holden department of biochemistry, university of wisconsin, madison, wisconsin, 53706 received 12 december 2018. World distribution of pantoea ananatis erwian eppo global database.
Investigation of a sugar n formyltransferase from the plant. Jun 02, 2016 pantoea agglomerans usually occurs in plants as an epi or endophytic symbiont, often as mutualist. Euglena gracilis, a unicellular phytoflagellate within euglenida, has attracted much attention as a potential feedstock for renewable energy production. Jun 08, 2015 pantoea ananatis is a highly versatile enterobacterium isolated from diverse environmental sources. Mlsa further indicated that the isolates represented a novel species, which was phylogenetically most closely related to pantoea ananatis and pantoea stewartii. May 27, 2014 pantoea ananatis is found in a wide range of natural environments, including water, soil, as part of the epi and endophytic flora of various plant hosts, and in the insect gut. Pantoea ananatis is ubiquitously found in the environment and causes disease on a wide range of plant hosts. This initial investigation has been extended to include an additional seven sequenced strains of p. Biotechnology consultation note to file bnf 000156. Darweesh et alinternational journal of chemtech research, 2016,98,pp 430437.
The type vi secretion system t6ss has been identified in several different bacteria, including the plant pathogenpantoea ananatis. Phylogenomic, pangenomic, pathogenomic and evolutionary. Gitaitis rd, walcott rr, wells ml, diaz perez jc, sanders fh, 2003. Results that are typical of the pantoea isolates are shown for nine isolates which are compared with the type strains of p. Characterization of pantoea ananatis isolated from garlic. Several of the identified bacteria, not known as onion pathogens in new york or elsewhere, caused various symptoms following introduction into. Structural elucidation and biological activity of acyl. Its genome was estimated at 4,869,281 bp, with 4,644 coding sequences cdss. Bacterial isolates from maize plants showing a leaf spot disease were identified through molecular and phenotypic traits, showing that the isolates belong to pantoea ananatis. All of the 28 pantoea isolates, except bud 10 not shown, were more similar to p. Prevelance and antibiotic susceptibility patterns of pantoeaspp. Analysis of the pantoea ananatis pangenome reveals factors.
This paper describes the chemical characterization of acylhsls produced by the worldwidespread phytopathogen pantoea ananatis serrano 1928 by using gas chromatographyelectron impact mass spectrometry. Genome comparisons to identify selected pathogenicity. Characterization of new bacterial leaf blight of rice caused. It is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in different parts of the world. Bacterial decays of onion bulbs cause sporadic and sometimes serious losses to onion allium cepa. Pantoea agglomerans usually occurs in plants as an epi or endophytic symbiont, often as mutualist. Draft genome sequence of pantoea ananatis strain 1. Pantoea agglomerans is a gramnegative bacterium that belongs to the family erwiniaceae it was formerly called enterobacter agglomerans, or erwinia herbicola and is an ubiquitous bacterium. Pdf center rot of onion, caused by the gramnegative, facultatively anaerobic bacterium, pantoea ananatis, was first described in georgia, usa, in.
The complete set of genes regulated by the eanir quorum sensing qs system in this strain is still. Allium cepa, burkholderia, dna primers, enterobacter, pantoea ananatis, bacteria, bulbs, databases, nucleotide sequences, onions, pathogens, polymerase chain reaction, new york abstract. Pantoea ananatis as an epiphyte pantoea ananatis is a common epiphyte on host and non. Darweesh department of biology, college of sciences, kufa university, najaf, iraq. Page designed through the cooperative efforts of interagency itis teams. Investigation of a sugar n formyltransferase from the. The bacterial onion pathogen pantoea ananatis, which lacks the virulence type iii and type ii secretion systems, induces cell death and necrotic symptoms on onions. Initially, it formed part of the erwinia herbicolaenterobacter agglomerans. Eight yellowpigmented, gramnegative, rodshaped, oxidasenegative, motile, facultatively anaerobic bacteria were isolated from onion seed in south africa and from an onion plant exhibiting centre rot symptoms in the usa. Amplified fragment length polymorphism analysis also placed the isolates into a cluster separate from p. Involvement of fenton chemistry in rice straw degradation by. Nevertheless, this species has also also been identified as a cause of diseases in a range of cultivable plants, such as cotton, sweet onion, rice, maize, sorghum, bamboo, walnut, an ornamental plant called chinese taro alocasia cucullata, and. Detection of pantoea ananatis in the early growing season is important for disease prediction and manage ment.
Genomic and secretomic insight into lignocellulolytic. Isolation and identification of pantoea ananatis from onion seed in south. Lignocellulolytic bacteria have revealed to be a promising source for biofuel production, yet the underlying mechanisms are still worth exploring. Pantoea ananatis amg 501 is a plant growthpromoting bacterium isolated from rice leaves. Isolation and identification of pantoea ananatis from onion seed in south africa article pdf available in seed science and technology 343. Itis taxonomy is based on the latest scientific consensus available, and is provided as a general reference source for interested parties. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf. Prevelance and antibiotic susceptibility patterns of pantoea spp. Some strains have proven effective as biological control agents and plantgrowth promoters, while other strains have been implicated in diseases of a broad range of plant hosts and humans. Integrative conjugative elements of the icepan family play. Pantoea ananatis defeats allium chemical defenses with a. Nevertheless, this species has also also been identified as a cause of diseases in a range of. Pairwise comparisons of lmg 2665t in the absence of ahls optical density od600. Genome sequence of pantoea ananatis lmg20103, the causative.
Comparative genomics of type vi secretion systems in. Genome sequence of pantoea ananatis sgair0210, isolated from outdoor air in singapore. Over recent years, impressive progress in its application to l. Involvement of fenton chemistry in rice straw degradation. Bacterial leaf blight pantoea agglomerans and bacterial center rot pantoea ananatis were. Pantoea ananatis strain aj355 taxonomy navigation pantoea ananas. Its unconventional nature lies in the fact that, unlike the majority of plant. This file contains the list of all strains of pantoea ananatis screened for the presence or absence type vi secretion system gene homologs. Following a request from the european commission, the efsa plant health panel performed a pest categorisation of pantoea stewartii subsp. The genome encompasses several cdss related to plant growth promotion, such as that for siderophore metabolism, and virulence genes characteristic of pathogenic pantoea spp. Crystal structure of phytoene desaturase crti from pantoea ananatis. Common name isynonym iother names i pantoea ananatis aj355 pantoea ananatis str. Unusual causes of peritonitis in a peritoneal dialysis.
Detection of pantoea ananatis, causal agent of leaf spot. Bacterial isolates from maize plants showing a leaf spot disease were identified through molecular and phenotypic traits, showing that the isolates belong. This is the first report of a disease of mexican maize caused by p. Apr 23, 2009 pantoea ananatis, a member of the enterobacteriacea family, is a new and promising subject for biotechnological research. Characterization of new bacterial leaf blight of rice caused by pantoea stewartii subsp. Genome comparisons to identify selected pathogenicity factors. Characterization of new bacterial leaf blight of rice. Characterization of pantoea ananatis isolated from garlic and. International journal of environment agriculture and biotechnologyissn.
Bacterial leaf blight pantoea agglomerans and bacterial center rot pantoea ananatis were isolated from 85 samples of onions from both counties. Pantoea ananatis is a gram negative plant pathogen that causes disease on a broad range of host plants. Pantoea ananatis, a cause of center rot of onion, is problematic. Over recent years, impressive progress in its application to lglutamate production has been achieved. Crtitype phytoene desaturases prevailing in bacteria and fungi can form lycopene directly from phytoene while plants employ two distinct desaturases and two cistans isomerases for the same purpose. It has been implicated in diseases of a wide range of agronomic crops worldwide, including onion, maize, rice and. We have identified a plasmidborne cluster of sulfurredox. Several of the identified bacteria, not known as onion pathogens in new york or elsewhere, caused various symptoms following introduction into healthyappearing onion foliage or bulbs. Characterization of pantoea ananatis isolated from garlic and shallot the new disease on garlic allium sativum and shallot a. Investigation of a sugar nformyltransferase from the plant pathogen pantoea ananatis daniel l. Nevertheless, genetic and biotechnological studies of pantoea ananatis have been impeded because of the absence of genetic tools for rapid construction of direct mutations. Genome sequence of pantoea ananatis strain amg 501, a. It has been implicated in diseases of a wide range of agronomic crops worldwide, including onion, maize, rice and pineapple, as well as a human disease. Genomic and secretomic insight into lignocellulolytic system.
Pantoea ananatis promotes growth of arabidopsis plants 15305 genetics and molecular research 14 4. Pantoea ananatis is a member of the family enterobacteriaceae and an enigmatic plant pathogen with a broad host range. Sep 14, 2017 pantoea ananatis is ubiquitously found in the environment and causes disease on a wide range of plant hosts. Pantoea ananatis is a species of gramnegative, rodshaped, aerobic or facultatively anaerobic, yellowpigmented bacteria belonging to the class gammaproteobacteria and the family enterobacteriaceae coutinho and venter, 2009. Bacterial strains used in this study are listed in additional file 1. Crystal structure of a sugar nformyltransferase from the plant pathogen pantoea ananatis. Goszczynska t, moloto vm, venter sn, coutinho ta, 2006. An 87 yearold female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. Pdf isolation and identification of pantoea ananatis. Pantoea ananatis, a member of the enterobacteriacea family, is a new and promising subject for biotechnological research.
Pdf isolation of pantoea ananatis from sugarcane and. Transcriptome profiling reveals the eanir quorum sensing. Comparative genomics of type vi secretion systems in strains. Pantoea ananatis is a member of the family enterobacteriaceae and is characterised by its ubiquity in nature and its frequent association with both plant and animal hosts. In gramnegative bacteria, the acylhomoserine lactones acylhsls are the main signaling substances employed in celltocell communication systems. However, it could be however, it could be a cause of opportunistic human infections, mostly by wound infection with plant material, or as a hospitalacquired infection.
In this study, rnasequencing rnaseq was used to identify the eanir regulon in lmg 2665t. Hosted by the usgs core science analytics and synthesis. Pdf pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. Pdf isolation and identification of pantoea ananatis from.
Its genome was estimated at 5,102,640 bp with 4,994 coding sequences, encompassing genes. May 01, 2009 summary pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. Among the plantinteracting species, pantoea ananatis, pantoea. It is regarded as an emerging pathogen based on the. The ecological diversity of this species may be attributed, in part, to the. The isolates were assigned to the genus pantoea on the basis of phenotypic and biochemical tests. This pathogen has a restricted lifecycle, overwintering in an insect vector before being introduced into susceptible maize. Genome sequence of pantoea ananatis strain amg 501, a plant. Maize plants inoculated with those isolates showed a pathogenic reaction. Lycopene is the precursor to provitamin a carotenoids, including. Frontiers phylogenomic, pangenomic, pathogenomic and. Pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide.